SHAKIBAEI M, SELIG K, DESOUZA P, VAN SICKLE D, STAHLMANN R; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 40 (abstract no. 1765).
Inst. of Anatomy, Freie Univ. Berlin, Berlin, GERMANY
Quinolones have the potential to induce Achilles tendon disorders (tendinitis, ruptures), but little is known about biochemical changes in tendons after quinolone exposure. We studied the Achilles tendons from quinolone-treated immature dogs biochemically. Ten to eleven weeks old dogs were treated orally for 5 days with 30 (n=5) and 200 (n=5) mg ciprofloxacin/kg b wt or with the vehicle (n=8). Because quinolone-like defects in joint cartilage have been observed in magnesium-deficiency, a further group was fed a magnesium-deficient diet for 6 weeks. Tendons were homogenized in lysis buffer and after centrifugation aliquots of the supernatant were studied in Western blots using antibodies directed against matrix proteins and integrins. Densitometric analysis of the immunoblots with anti-collagen type I, anti-elastin, anti-fibronectin, and anti-integrins antibodies showed a significant reduction of all proteins. For example, the collagen I concentrations were 3,315 +/- 35 (controls), 1,710 +/- 254 (30 mg/kg), 2,520 +/- 42 (200 mg/kg) and 1,800 +/- 311 in the magnesium-deficient dogs (mean +/- sd, arbitrary units). Differences between concentrations in controls and in all treated groups were statistically significant (p< 0.01; t-test). Similarly, relative concentrations of other proteins in tendons from ciprofloxacin-treated dogs decreased by 73% (elastin), 88% (fibronectin), and 96% (Beta1-integrin) when compared to control samples (data from low-dose group only). A very similar pattern of protein alterations was detected in magnesium-deficient samples. In conclusion, rather low doses of a fluoroquinolone or a dietarily-induced magnesium deficiency induce identical biochemical alterations in canine tendons. These findings further support the hypothesis that quinolone-induced toxic effects on connective tissue structures are due to the magnesium-antagonistic effects of these antibacterial agents.
Publication Types:
Keywords:
- Achilles Tendon
- Animals
- Anti-Bacterial Agents
- Antigens, CD29
- Blotting, Western
- Cartilage, Articular
- Ciprofloxacin
- Collagen
- Dogs
- Extracellular Matrix Proteins
- Fibronectins
- Fluoroquinolones
- Integrins
- Joint Diseases
- Magnesium
- Magnesium Deficiency
- Proteins
- Quinolones
- Tendinopathy
- immunology
Other ID:
UI: 102244628
From Meeting Abstracts