HERTOGS K, DEVROEY V, VANDENEYNDE C, MILLER V, DEHERTOGH P, VANCAAUWENBERGE A, BLOOR S, ALCORN T, LARDER B; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 464 (abstract no. 425).
VIRCO, Mechelen, BELGIUM
We present an analysis of over 5,000 samples from US and European routine clinical practice, tested last year by commercial CLIA-approved phenotypic and/or genotypic methods (Antivirogram[TM], VircoGEN[TM]). 85% of these had phenotypic resistance to at least one antiretroviral class (NRTI, NNRTI or PI). Combined resistance to drugs from 2 or 3 classes was seen respectively in 20% and 70% of samples with any phenotypic resistance. Class-specific MDR was detected in 5%, 48% and 57% of the NRTI-, NNRTI- or PI-resistant isolates. The following overall frequencies were observed for common mutations in RT: 215Y (33%) (vs 215F (8%) or 215D/C (1.4%)), 184V (38%), 103N (20%) and 181C (14%). The following overall frequencies were observed for common protease mutations: 30N (6%), 48V (4%), 82A (14%) (vs 82S/ F/T (3%)) and 90M (33%). In RT, a 151M mutation was seen in 1.5% of the isolates. Nine different types of amino acid insertions were identified in the codon 68 to 70 region of RT. These differed in phenotypic impact and in all represented 1.2% of the total. Several novel amino acid substitutions were identified at different RT condons (e.g., 101, 103, 181, 184), or in the protease (e.g., 88 and amino acid insertions). Most interestingly were the novel mutational patterns associated with phenotypic 3TC resistance (in an AZT resistance mutational background), with specific NNRTI cross-resistance (in a 190A background) and with PI cross-resistance (in a 54V background). It is anticipated that the continued use of combined phenotypic and genotypic resistance testing technologies in routine clinical practice will provide further insights both into the prevalence of common and rare resistance profiles and the emergence of new resistance pathways.
Publication Types:
Keywords:
- Amino Acid Substitution
- Codon
- HIV-1
- Lamivudine
- Mutation
- Prevalence
- Zidovudine
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102245748
From Meeting Abstracts