SCAGLIONE F, PANNACCI M, LUCINI V, GROSSO S, COLLEONI F, CARONZOLO D; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. E-1994.

University of Milan, Milan, Italy.

BACKGROUND: Chlamydia pneumoniae (Cp) persistence after antibiotic treatment could be one of the causes of atherosclerosis and asthma. Aim of this research is the development of therapeutic association able to eradicate Cp. METHODS: Telithromycin (Tel) and levofloxacin (Lev) were used alone or in association with dexamethasone (Dex). Tests were performed using the Cp antibiotics MIC evaluation culture protocol with slight modification. HeLa cells, grown to confluence on 12 mm coverslip, were inoculated (5x10[4] IFU) with Cp (strain AR-39) and incubated with antibiotics alone or in combination with Dex. Presence of Cp elementary bodies will pointed out by immunofluorescence 72 h after. Photos (40x ocular) of four different fields were taken for each slide. Two different operators evaluated number of cells and of Cp IFU in double blind. Statistical analysis was performed using ANOVA. RESULTS: Using both antibiotics alone, number of infected cells decreases significantly vs control. Lev reduced percentage of infected cells vs control about to 72% at 0.25 microg/ml (p<0.01), 62% at 0,5 microg/ml (p<0.001), 54% at 2,5 microg/ml (p<0.001) and to 43% at 5 microg/ml (p<0.001). Tel reduced about to 63% at 0.05 microg/ml (p<0.001), 54% at 0,1 microg/ml (p<0.001), 49% at 0,5 microg/ml (p<0.001) and to 29% at 1 microg/ml (p<0.001). Dex (1 micro-M) reduced about to 48 % (p<0.001). Association of antibiotic with dexamethasone produce a significant decrease of infected cells vs they used alone. Lev plus Dex reduced percentage of infected cells vs control about to 27% at 0.25 microg/ml (p<0.001), the 25% at 0,5 microg/ml (p<0.001), the 23% at 2,5 microg/ml (p<0.001) and the 19% at 5 microg/ml (p<0.001). Tel plus Dex reduced about to 27% at 0.05 microg/ml (p<0.001), 26% at 0,1 microg/ml (p<0.001), 20% at 0,5 microg/ml (p<0.001) and to 13% at 1 microg/ml (p<0.001). CONCLUSIONS: Combination of antibiotics and Dex (or others corticosteroids) could be clinically evaluated as appreciable therapeutic opportunity for treatment of Cp infection.

Publication Types:

• Meeting Abstracts

Keywords:

• Anti-Bacterial Agents
• Chlamydophila pneumoniae
• Dexamethasone
• Humans
• In Vitro
• Ketolides
• Microbial Sensitivity Tests
• Motor Activity
• Ofloxacin
• telithromycin

Other ID:

• GWAIDS0025304

UI: 102264928

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