BRANDT C, LUNDGREN JD, LUND SP, FRIMODT-MOLLER N, CHRISTENSEN T, BENFIELD T, ESPERSEN F, HOUGAARD D, OSTERGAARD C; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. B-1513.
Statens Serum Institute, SSI, Copenhagen, Denmark.
BACKGROUND: Attenuation of the inflammatory response with leukocyte blocking agents has shown promising results in animal models of bacterial meningitis. However outcome and braindamage has not been studied. METHODS: Rats were randomized to receive the selectin blocker fucoidin (25 mg/kg i.p x 3 d.) from time of infection (0h, n=45) or no fucoidin (n=29). Animals were injected intracisternally with 3 x 10[3] CFU S.pneumoniae type 3, treated with ceftriaxone 28 h postinfection and followed for 7 days. Blood and CSF samples were obtained 24 h postinfection. Brain damage was histologically assessed in 50 brains. Randomization of the trials was blinded to the evaluators. RESULTS: Fucoidin treatment increased lethal outcome (21/45 vs. 5/29 in the control group, logrank test p=0.011). Cerebrospinal fluid (CSF) pleocytosis was attenuated in the fucoidin treated group (median 400 vs 1000 x 10[6] cells/L, Mann-Whitney P=0.003). No differences in bacterial titers from CSF (median 2.1 x 10[5] vs 2.6 x 10[5] CFU/ml) or blood (median 9.5 x 10[2] vs 5.4 x 10[2)] were observed. Blood WBC were similar in the two groups (median 4.7 vs 4.8 x 10[9] cells/L). Brain damage score was not different in the two groups (median 0/180 for both groups). CONCLUSIONS: Pretreatment with fucoidin increases mortality in a rat model of experimental meningitis.
Publication Types:
Keywords:
- Animals
- Bacteremia
- Brain
- Ceftriaxone
- Communicable Diseases
- Disease Models, Animal
- Infection
- Leukocytes
- Leukocytosis
- Meningitis
- Meningitis, Pneumococcal
- Models, Biological
- Muridae
- Polysaccharides
- Rats
- Rats, Wistar
- Selectins
- blood
- cerebrospinal fluid
- fucoidan
- immunology
Other ID:
UI: 102265296
From Meeting Abstracts