AFELTRA J, VITALE RG, MEIS JF, VERWEIJ PE; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. M-1532.

Department of Medical Microbiology, University Medical Center, Nijmegen, Netherlands.

BACKGROUND: We investigated the in vitro activity of trimethoprim (TMP), pyrimethamine (PMT), 7 sulphonamides including sulphamethoxazole (SMX), cotrimoxazole (SXT), dapsone (DAP), sulfisoxazole (SSX), sulfamethizole (SMT), sulfadiazine (SDZ), sulphamethoxypyridazine (SMP), and 8 antiparasitic agents including chloroquine (CH), primaquine (PR), quinacrine (QR), quinine (QN), thiabendazole (TH), metronidazole (MT), artemisin (AR) and pentamidine (PN) against 42 clinical isolates of Cn. METHODS: Susceptibility testing was performed by broth microdilution method using two media (RPMI 1640 with 2%glucose and yeast nitrogen base [YNB] buffered with MOPS) incubated at 35 degrees C for 48 h with shaking. The inoculum size was 10[4] CFU/ml, and spectrophotometric determination of a 50% growth inhibition endpoint (MIC-2) was used. Final concentrations of the agents were 64 to 0.06 mg/l for TMP; 320 to 0.31 mg/l for SMT; 16/320 to 0.016/0.31 mg/l and 64/320 to 0.06/0.31 mg/l for SXT; 2 to 0.002 mg/l for PMT and PR; 8 to 0.008 mg/l for DAP; 640 to 0.62 mg/l for SMP; 320 to 0.31 mg/l for SSX, SMT and SDZ; 32 to 0.031 mg/l for CH, QN and PN; 16 to 0.21 mg/l for QR and AR; 128 to 0.12 for MT and TH. RESULTS: TMP, SSO, SMT, PMT, DAP, CH, PR, QR, QN, MT exhibited no activity in vitro. The MIC[50] of SMX, SXT, SDZ, SMP, TH were 80, 80, 320, 160, and 16 mg/l, respectively, in YNB and of AR and PN 4 and 1 mg/l, respectively, indicating in vitro activity. The antifungal activity was fungistatic. Sulphonamides were more active in YNB than in RPMI because RPMI contains high amounts of para-aminobenzoic acid which inhibits the activity of sulphonamides. PN and AR were more active in RPMI than YNB. CONCLUSIONS: SMX, SXT, SDZ, SMP, TH, AR and PN showed fungistatic antifungal activity against Cn, however more in vitro investigation, animal model and clinical trials are necessary to confirm these data.

Publication Types:

• Meeting Abstracts

Keywords:

• Antifungal Agents
• Antigens, Fungal
• Antiparasitic Agents
• Cryptococcosis
• Cryptococcus neoformans
• In Vitro
• Microbial Sensitivity Tests
• Sulfamethoxazole
• Trimethoprim
• immunology

Other ID:

• GWAIDS0026912

UI: 102266536

From Meeting Abstracts