PARSONNET J, DELANEY M, DUBOIS A, MODERN P, WIELAND-ALTER W, HANSMANN M, SEYMOUR J, WILD J, ONDERDONK A; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. B-1437.
Dartmouth-Hitchcock Med Ctr, Lebanon, NH.
BACKGROUND: Development of menstrual TSS requires colonization with TSST-1-pos S. aureus and absence of Ab to TSST-1. 3012 women were screened for Ab to TSST-1 and carriage of TSST-1-pos S. aureus.1 year later 311 subjects from the initial cohort were recalled based on S. aureus carriage and Ab status and followed for an additional 8-11 months. METHODS: Samples collected from nares, anus, and vagina were cultured by techniques optimal for recovery of S. aureus. Serum Ab levels to TSST-1 were determined by ELISA. RESULTS: 21% (3/14) of subjects with TSST-1-pos S. aureus in the vagina during the screening period were colonized vaginally at some time during the recall (6% of visits), whereas 13% of subjects initially colonized with toxigenic S. aureus in the nose or anus were colonized vaginally during the recall. Previous colonization with toxigenic S. aureus in the nares and/or anus was the best predictor of subsequent colonization at any of the 3 body sites tested (48% of return visits). 3 subjects were found to carry both toxigenic and non-toxigenic S. aureus on more than 1 return visit. Colonization with a TSST-1-pos strain was associated with high levels of serum Ab, higher than among women without S. aureus or with a negative strain. In the period between the screening and recall visits, 4 of 75 subjects with no initial Ab became seropositive (titer >1:16) and 1subject became positive during the recall phase, at which time she was colonized with a toxigenic strain (seroconversion rate 7%). None of these subjects experienced TSS-like symptoms. CONCLUSIONS: Vaginal colonization with TSST-1-pos S. aureus was not a good predictor of continued colonization, whereas nasal and anal colonization did predict continued colonization. Seroconversion to TSST-1 was not uncommon in subjects without initial Ab and was asymptomatic in all subjects.
Publication Types:
Keywords:
- Antibodies
- Antibodies, Anti-Idiotypic
- Antibodies, Bacterial
- Bacterial Toxins
- Enterotoxins
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunoglobulin G
- Menstruation
- Nasal Cavity
- Shock, Septic
- Staphylococcus aureus
- Superantigens
- Vagina
- enterotoxin F, Staphylococcal
- immunology
Other ID:
UI: 102268021
From Meeting Abstracts