MIRANDA CD; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. C2-2241.
Univ. Catolica del Norte, Coquimbo, Chile
Intensive fish farming in Chile has resulted in massive use of antibiotic agents for treatment and prevention of bacterial infections. Oxytetracycline, is the most frequently used antibacterial in the industry, as a result oxytetracycline resistant pathogens are a significant issue. We examined 26 oxytetracycline-resistant Gram-negative bacteria recovered from different Chilean salmon farms. In addition, 2 oxytetracycline resistant Yersinia ruckeri strains, isolated from Enteric Redmouth Disease outbreaks, were included. The MIC[50] and MIC[90] for oxytetracycline were 1024 and 2048 microg/ml, respectively for the 28 isolates. The isolates were analyzed using DNA-DNA hybridization to characterize the presence of tet (A)-(E), (G), (H) genes. Six (21%) of the isolates (4 Pseudomonas fluorescens, 1 Pseudomonas spp. and 1 Escherichia coli) carried the tet(A) gene; 4 (14%) of the isolates (Serratia liquefaciens, Pseudomonas pseudoalcaligenes, Enterobacter sakazakii and Brevundimonas vesicularis) carried the tet(B) gene; 2 (7%) of the isolates (Moraxella spp. and Acinetobacter radioresistens), carried the tet(H) gene; and 1 (3.5%) isolate (Aeromonas hydrophila) carried the tet(E) gene. No isolate carried the tet(C), tet(D) or tet(G) genes. The 15 isolates which did not hybridize with the 7 tet genes are primarily non-fermenting and represent genera which have not been previously studied. In contrast, in other studies of fish farm associated water bacteria, the tet(A)-(G) genes have been found in the majority of the isolates examined. Our results suggest that there may be some differences between the bacteria and the tet genes they carry from Chilean salmon farms compared to fish farm associated water bacteria previously described in other parts of the world.
Publication Types:
Keywords:
- Animals
- Anti-Bacterial Agents
- Bacteria
- Chile
- Escherichia coli
- Fishes
- Gram-Negative Bacteria
- Microbial Sensitivity Tests
- Oxytetracycline
- Salmon
- Tetracycline Resistance
Other ID:
UI: 102269698
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