CORMICAN MG, DELAPPE NP, CORBETT-FEENEY G, FANNING S, OHALLORAN F; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. C2-58.
National University of Ireland, Galway, Galway, Ireland
Shigella sonnei is a leading cause of gastroenteritis in both developing and industrialised countries. Various phenotypic and genotypic methods have been used to type S. sonnei isolates, including phage typing, antimicrobial resistance typing (ART), pulsed field gel electrophoresis (PFGE), random amplification of polymorphic DNA (RAPD) and plasmid profiling. Sixty-seven S. sonnei isolates from various counties in Ireland were analysed using PFGE and ART, while phage typing (n=8), plasmid profiling (n=28), and integron analysis (n=14) was carried out on subsets of strains. PFGE divided the isolates into two major clusters, A (n= 53) and B (n=14). There were seven different resistance profiles detected, with resistance to ampicillin, streptomycin(S) and sulphonamide (Su) (associated exclusively with PFGE A), being the most common (76%). Resistance to S, Su, tetracycline and trimethoprim was common within PFGE B (78%). Two PFGE A strains phage typed were PT9, while the PFGE B strains typed as PT6 (n=4), and PT50 (n=1) and RDNC (n=1). There were several different plasmid profiles among the strains analysed. Two different plasmid profiles were observed among PFGE A, ASSu strains. Integron analysis of a subset of isolates (n=14) revealed that at least 1 isolate contained a complete class 1 integron structure while 10 isolates contained a gene encoding integrase 2. Six isolates which were resistant to trimethoprim by phenotypic methods contained an amplicon for a DHFR gene which confers resistance to trimethoprim. Our data demonstrate very limited diversity among S. sonnei in Ireland by PFGE, but greater discrimination can be achieved by a use of a variety of typing methods.
Publication Types:
Keywords:
- Bacteriophage Typing
- Dysentery, Bacillary
- Electrophoresis, Gel, Pulsed-Field
- Integrons
- Ireland
- Plasmids
- Random Amplified Polymorphic DNA Technique
- Shigella sonnei
- genetics
Other ID:
UI: 102270392
From Meeting Abstracts