Komninakis SC, Fukumori LM, Alcalde R, Cortina MA, Brito A, Duarte AJ, Casseb J; International Conference on AIDS (15th : 2004 : Bangkok, Thailand).
Int Conf AIDS. 2004 Jul 11-16; 15: abstract no. A10500.
FMUSP, Sao Paulo, Brazil
Background: HIV-1 subtype B predominates in Brazil, and the V3 loop tip presents two major signature sequences: B-GPGR and B"-GWGR, the latter almost unique to Brazil. Aim: To analyze the occurrence of these variants in our cohort, using serological and molecular tools. Material and Methods: A total of 87 HIV-infected individuals who have been followed in follow-up in our HIV Out-Clinic at the HC/FMUSP were tested by anti-V3 serology and molecular methods (RFLP-Fok 1) and direct DNA sequencing of the env gene. Results: Of the 87 samples evaluated through the EIA-solid phase, 46 (53%) reacted for B-GPGR, 31 (36%) B"-GWGR and 10 (11%) were indeterminated for synthetic peptides set. The 10 samples that were characterized as indeterminate were analyzed through the EIA-inhibition and, 5 reacted for B-GPGR, 4 for B"-GWGR and 1 indeterminated. The 87 samples were analyzed for RFLP using Fok 1, 25 samples were B-GPGR, concordant to EIA-solid phase, 11 were B"-GWGR concordant, 20 were indeterminate and 9 samples were not amplified by primers pair used. Of the 87, 61 samples which were possible to have nucleotide sequences, 20 GPGR (13 were concordant in EIA), 19 sequences GWGR (of these, 13 were concordant in EIA) and 22 samples showed different amino acids in the tip of the V3 loop. Conclusion: In the subtype B variant B"-GWGR identification, is suggested to use serotyping (EIA-inhibition), and direct DNA sequencing. Support: FAPESP ndegrees 00/12763-9; FAPESP ndegrees 00/11868-1
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Base Sequence
- Brazil
- Genes, env
- HIV Infections
- HIV Seropositivity
- HIV-1
- Immunoenzyme Techniques
- genetics
- methods
Other ID:
UI: 102276348
From Meeting Abstracts