Chequer-Fernandez SL, Rodrigues CA, Campos-Melo DL, Pilotto JH, Morgado MG; International Conference on AIDS (15th : 2004 : Bangkok, Thailand).

Int Conf AIDS. 2004 Jul 11-16;15:abstract no. MoPeC3442.

Laboratory of AIDS and Molecular Immunology, Depto of Immunology-IOC/ Oswaldo Cruz Foundation, Rio de Janeiro, Brazil

Background: HIV-1 presents a high genetic diversity, with at least 11 subtypes/sub-subtypes and 15 CRFs already described. The implications of such variability for pathogenicity and efficacy of antiretroviral therapy are still under evaluation. Methods: To investigate the prevalence of the HIV-1 subtypes in Rio de Janeiro, Brazil and their potential implications on the antiretroviral therapy, we analyzed 170 HIV-1 recently infected individuals from Rio de Janeiro City, regarding genetic subtypes and genotyping of viral resistance. All patients were clinically evaluated and submitted to plasma viral load and T CD4+ determination each four months. The HIV-1 subtyping was determinated by env heteroduplex mobility assay and the genotyping by the sequencing of amplified PCR nested fragments corresponding to the pol gene. Results: The majority of HMA subtyped patients were classified as HIV-1 subtype B (73%), including the B" variant (14%), followed by subtype F (10%) and subtypes D and C (1,2% each). Fourteen individuals hadn't indication to start antiretroviral therapy. The genotyping of HIV-1 resistance performed in 56 (29%) individuals with indication to initiate antiretroviral therapy, showed the mutations T69S (11,5%), E44D and K70R (3,8% each) against transcriptase reverse inhibitors and the L90M (6%)mutation against protease inhibitors. Conclusions: No significant increase of the CD4 counts was observed in response to antiretroviral therapy independently of the subtype infection. Viral load was reduced along the treatment in both groups infected with B (P<0.0017) and non-B (P<0.0313) HIV-1 virus. However, for those infected with the B" variant, the reduction on the viral load was not quite significant (P = 0.0645). Virus resistance mutation profiles of PRO and RT regions before and after antiretroviral treatment are being determined. In spite of the small sample size due to the reduced frequency of non-B subtypes in RJ, among others, slight differences are being observed between B and B" infections in response to antiretroviral therapy.

Publication Types:

• Meeting Abstracts

Keywords:

• Adult
• Anti-HIV Agents
• Brazil
• CD4 Lymphocyte Count
• Female
• Genes, env
• Genes, pol
• Genetic Variation
• Genotype
• HIV Infections
• HIV Reverse Transcriptase
• HIV Seropositivity
• HIV-1
• Humans
• Male
• Middle Aged
• Mutation
• Polymorphism, Genetic
• Prevalence
• Reverse Transcriptase Inhibitors
• Viral Load
• drug therapy
• genetics
• virology

Other ID:

• GWAIDS0035527

General Notes:

• Meeting held in: Thailand

NLM Unique ID: 9870004
UI: 102279743